Fluorescent Microscopy/Hyperspectral Imaging Facility

photoThe imaging laboratory consists of novel and advanced fluorescence stereo and dipping microscopes each supported by a computer and extensive software designed to manage the data output.

The MVX-10 macroscope enhances our ability to capture brightfield and fluorescent images (both static and video) of live corals and the symbiotic and parasitic animals associated with them.

The custom outfitted Olympus BX51 is equipped with a PARISS (Prism and Reflector Imaging Spectroscopy System) advanced hyper-spectral imaging device that simultaneously collects wavelengths between 300nm and 900nm. The system consists of an imaging spectrometer coupled to a CCD camera that can capture spectral signatures from the inherent auto-fluorescence of corals.

The dipping objectives range from 2X-40X in magnification and provide close-up views of live coral specimen enhancing collection of fluorescent emission spectra from specific coral morphological features. The spectral data is used in both diagnostics and discovery research.

Both systems are tied to battery back-up devices and the laboratory is maintained at constant temperature and low-humidity.